广州市生鲜鸡中肠炎沙门菌的监测及耐药基因的分子鉴定

doi:10.11843/j.issn.0366-6964.2018.02.018

Abstract

Abstract:

This experiment was conducted to investigate the prevalence of Salmonella, the distribution of serotypes and the antibiotic resistance of Salmonella enteritidis, and resistant genes they carried, in chicken of Guangzhou city. According to the national standard GB4789.4-2016, the chicken samples were collected. Salmonella serotypes were determined using diagnostic sera from S&A, Thailand. The antibiotic resistance of Salmonella enteritidis was determined by using the Kirby-Bauer method. The resistance gene of Salmonella enteritidis was detected by PCR. In 316 samples of chicken, the positive rate was 76.9%. A total of 23 serotypes were identified. The main serotypes include Salmonella Agona (19.8%), Salmonella Kottbus(14.0%), Salmonella Mbandaka (11.9%), Salmonella Kentucky(10.3%), Salmonella enteritidis (7.8%) and Salmonella Branederup (7.4%). The resistance rates of Salmonella enteritidis to nalidixic acid, peptidylsulfanilamide, ampicillin, streptomycin, tetracycline, gentamicin, cefotaxime and ceftazidime were 100.0%, 79.0%, 57.9%, 36.8%, 21.0%, 21.0%, 15.8% and 5.3%, respectively. The multiple antibiotic resistance rate was 68.4%. The mutation rates of gyrA, gyrB and parC were 94.7%, 73.7% and 15.8%, respectively. The most main mutations were Phe-414→Ser (73.7%), followed by Asp-87→Gly (42.1%),Asp-87→Trp(31.6%) and Ser-83→Trp(21.1%). The detection rate of quinolone-resistant plasmids was low. The detection rates of bla_TEM and bla_CTX-M were 42.1% and 10.5%, respectively. The pollution rate of Salmonella in chicken of Guangzhou city is high and the serotype is complex. Salmonella enteritidis are resistant to Nalidixic acid, peptidylsulfanilamide, ampicillin. Multiple antibiotic resistance is high. The mutation rate of the gene in the quinolone resistance-determining region is high, which is highly consistent with the resistance of Salmonella to quinolones.

CLC Number:

R378

SUN Peng-hao, QIU Rong-chao, LI Shu-ning, ZHENG Shuang-shuang, YANG Wei-cong, ZHANG Li-na, FU Ying, ZHAN Ze-qiang, ZHANG Jian-min, LIAO Ming, QU Xiao-yun. Monitoring of Salmonella Enteritidis and Molecular Identification of Antibiotic Resistance Gene in Fresh Chicken of Guangzhou City[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(2): 388-395.

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Monitoring of Salmonella Enteritidis and Molecular Identification of Antibiotic Resistance Gene in Fresh Chicken of Guangzhou City

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